Degradable Self-Assembling Dendrons for Gene Delivery Experimental and Theoretical Insights into the Barriers to Cellular Uptake

Anna Barnard, Paola Posocco, Sabrina Pricl, Marcelo Calderon, Rainer Haag, Mark E. Hwang, Victor W. T. Shum, Daniel W. Pack, and David K. Smith | University of York, University of Trieste, Freie Universitat Berlin, University of Illinois

In this study, multilevel modeling was used to demonstrate that complete dendron degradation would be necessary for effective DNA release. The choosen integrated experimental and theoretical approach has provided a unique insight into the way in which gene delivery vectors can approach cellular barriers to gene delivery. The entire MD simulation and data analysis procedure was optimized by integrating molecular modeling tools in modeFRONTIER.